The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. You should get comparable results from top and bottom reads using EC50's to compare reads. • Tel. es •. The advantage of using labeled probes is detection specificity. All associated StakMax® stackers. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode. Flurescence intensities were read using a Spectramax M5 microplate reader (Molecular Devices, Sunnyvale, CA, USA). The SpectraMax M5 utilizes a single monochromator design for UV/VIS Absorbance,. HTRF-compatible SpectraMax Multi-Mode Readers. Microplate Readers Molecular Devices Spectramax iD3 Microplate Reader. 2. , USA). Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. Representative data from the SpectraMax i3x Multi-Mode Microplate Reader for both MycoAlert and MycoAlert PLUS assays are shown in Figure 3. Molecular Devices FlexStation™ 3 Multi-Mode Microplate Reader (Recent Model) with One Year Warranty (Svc Contract Available) Molecular Devices SpectraMax™ M5e Microplate Reader with 90 Day Depot Warranty (Svc Contract Available). The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. 1. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 1. Compatible automation solutions for SpectraMax Readers. Note: In this user guide, all references to SpectraMax Multi-Mode. Plasma Tau was determined by high-performance liquid chromatography (Shimadzu ®, model LC 10AD) using a Shimadzu model RF 535 fluorescence detector. 1. SpectraMax ® M5 Multi-Mode Microplate Reader Transcreener ® ADP. Microplate Reader Shelf. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. anisotropy was measured on SpectraMax M5 microplate reader with 485 nm excitation and 535 nm emission after 60 min incubation at room temperature. 1. The dual monochromator optics allow the widest range of applications to be. SpectraMax iD3, iD5, i3x, i3, M3, M4, M5, M5e, SpectraMax L, 6186 Luminescence FlexStation 3. Fifteen different automated tests are provided. As readout, fluorescence was measured at Ex/Em = 540/590 nm using a SpectraMax M5 microplate reader with SoftMax Pro v7 (Molecular devices) after 4–6 h of incubation in normal cell-culture medium. When the plate was read on the SpectraMax M5 microplate reader, an enzyme concentration range of 0. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. The detection of lactate dehydrogenase releaseBriefly, algae were harvested, centrifuged, and re-suspended with an equal volume of methanol at the end of the experiment. A new microplate luminometry for the toxicity bioassay of chemicals on firefly luciferase, was developed using the multifunctional microplate reader (SpectraMax M5) to measure the luminous intensity of luciferase. The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume. SpectraMax® Microplate Readers and SoftMax® Pro Software. B: Selection of Excitation Wavelength. 5 Dual-luciferase reporter assay. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. #SpectraMax L Config) Methods TIGIT/CD155 Blockade Bioassay. 5 units/mL yielded a delta mP of 303, with a Z’ factor of 0. Microplate Readers Biotek Epoch Microplate. When a The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. Molecular Devices LMax II Luminometer Luminescence Microplate Reader w WARRANTY $2,450. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. book Page 7 Wednesday, July 7, 2010 2:09 PM. They were allowed to attach and grow overnight in a 37°C, 5% CO 2 incubator. 00. The cuvette must be in place when you read the plate. View the Molecular Devices website. 87. 6-384. SpectraTest Validation Plates form Molecular Devices such as ABS1 absorbance, FL1 fluorescence & LM1 luminescence validation plates are the valuable tools for verifying that absorbance, fluorescence, and luminescence-capable microplate readers are operating correctly for GLP and GMP laboratories. When a FlexStation® 3 Multi-Mode Microplate Reader. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. Standard features include a. Published Dec 16, 2020 | Updated Dec 16, 2020. Table of Contents. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. Spectramax M5 Microplate Reader, supplied by Danaher Inc, used in various techniques. 7 cells were co-transfected with 0. SpectraMax iD5 Multi-Mode Microplate Reader. A: Microplate Preparation. Bottom-reading is usually recommended for cell-based assay with adherent cells. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax. As. microplate reader. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini EM Reader, users never have to worry about not having the right set of filters. 6-384 (96- and 384-well with pipetting) 6-384. When the plate was read on the SpectraMax M5 microplate reader, an enzyme concentration range of 0. • SpectraMax Mini Multi-Mode Microplate Reader (AccuBlue HiRange only) • SpectraMax® M2/M2e/M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate Reader • FlexStation® 3 Multi-Mode Microplate Reader • Gemini™ EM/XPS Fluorescence Microplate Readers • FilterMax™ F3/F5 Multi-Mode. Microplate Reader, Multimode Tecan Infinite M200 Pro Nanoquant M Nano+ Dual-Mode Microplate Reader 30050303. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. Figure 1. Self Service Available. book Page 1 Wednesday, October 12, 2011 4:56 PMSpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. MOLECULAR DEVICES SpectraMax M5 Microplate Reader For Sale. Table 1. AlphaScreen assays are compatible with our SpectraMax i3x, SpectraMax i3, and SpectraMax Paradigm Multi-Mode Microplate readers with the Alphascreen Detection Cartridge options. A: Microplate Preparation. The cells were then treated to induce apoptosis. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. The dual monochromator optics allow the widest range of applications to be utilized for bior. Optical absorbance. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well. ATP standard curves read on SpectraMax microplate readers. Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. The absorbance was measured at 570 nm using a SpectraMax M5 microplate reader (Molecular Devices, Sunnyvale, CA). Figure 1. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. For more sample throughput, the system can Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, VersaMax reader, FlexStation 3 reader I need to reinstall the USB driver for my plate reader. M5 SpectraMax M5e Absorbance X X X X Fluorescence intensity X X X X Fluorescence polarization XX Time-resolved fluorescence XX X Luminescence x x X X Note: In this user guide, all references to SpectraMax Multi-Mode Microplate Readers include the M3, M4, M5, and M5e models. TR-FRET assay. Urea-induced unfolding of rA1AT-WT and the Q9N mutant was measured using a SpectraMax. We would like to show you a description here but the site won’t allow us. Fluorescence Microplate Reader Comparison Chart. based microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. Recommended Optics Wavelength (nm) Wavelength. HeLa cells were plated at 20,000 cells per well in 100 µL per well in a black, clear-bottom microplate. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. The raw data from each individual experiment were normalized to vehicle-treated HK-2. l. 315 can't find zero order. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. This monochromator-based instrument allows users to select optimal wavelengths for any chosen fluorophore without the need to purchase additional filter sets. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. . Microplate Readers are modular and . 7°C and the bottom to 36. com. The dual monochromator optics allow. The plate reader can measure multiple readouts, including UV and visible absorbance. This integrated system provides users with a multi-detection platform capable of increasing the liquid handling throughput and flexibility for biochemical- and cell-based kinetic assays. Endpoint, kinetic, spectrum and area-well scanning read types and the PathCheck®. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Click SoftMax Pro Software User Guide. Efects of luciferase concentration, luciferin concentration, ATP concentration, pH, temperature, and reaction time on the. VersaMax Microplate Reader An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. It measures the optical density (OD) of samples in a microplate or in a cuvette at aThe cells were then washed three times with PBS, and the fluorescent intensity of the cells was measured with a SpectraMax M5 microplate reader (Molecular Devices, USA) at an excitation wavelength of 488 nm and emission wavelength of 525 nm. Bookings are free, but you will need to use iLab to book. 1. The PBS solution (10. Microplate Readers are modular and . SpectraMax® ABS. The Spectr. The SpectraMax® M5 Multi-Mode Microplate Reader is ideally suited for assay development and high-throughput screening using IMAP assays. The system. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). 00 USD. played in a grid format that corresponds to the wells in a microplate (all instruments) or individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). Other technologies such as imaging. If you do not have an iLab account you must first make one: Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. When the user applies a set temperature of 37°C, the top is heated to 37. The BioTek Synergy H1 multimode microplate reader is modular and upgradable to include variable-bandwidth monochromator optics for fluorescence excitation and emission wavelength selection, plus luminescence and UV-Vis absorbance. Download PDF. Ltd. The day before assay, assay buffer was prepared as described in the product technical manual. 1–0. Microplate Data Acquisition and Analysis Software, users are now able to extend the ease-of-use of their typical plate reader applications to cell-based imaging and western blot detection. Shop now. Parallel negative control assays had primary, secondary, or both antibodies omitted. IMAP phosphodiesterase assays on SpectraMax Multi-Mode Microplate Readers. 4. 1. These robust readers have been placed in labs from Antarctica to. Insert the cuvette into the chamber, making sure that the clear sides are to the left and right (facing the instrument). Molecular Devices | Life Science Applications | Bioanalytical. Microplate Readers include the M3, M4,. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. FlexStation™ II. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. 8 µg pNF-κB-After 3 h of incubation at 37 °C, the medium was gently removed, and a 150-μL aliquot of DMSO was added to each well. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. The SpectraMax ® iD5 Multi-Mode Microplate Reader includes an absorbance detection mode optimal for protein quantitation assays. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. 5 Compatible microplate readers FP Plate Reader SpectraMax® M5/M5e Multi-Mode Microplate ReaderSpectraMax M5 Multi-Mode Microplate Reader; Method. In this application we demonstrate how PDE enzyme dilution and inhibition curves are performed with IMAP Technology using the SpectraMax ® i3, SpectraMax ® Paradigm ®, SpectraMax ® M5, and FlexStation ® 3 Multi-Mode Microplate Readers with SoftMax ® Pro Software. For example,it is already turned on and the screen should look like this with “SpectraMax M5” showing as connected in the top left: 7. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. 2. Wide range of applications. This is equivalent to having 511 built-in filters,The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplate. 313 reference gain check fail. KEY FEATURES • Five. With optimized reagents, option to operate the readers. A serial dilution of IgG standards was performed, using XP Media/L-glutamine as the diluent, to concentrations from 2. Fluorescence from the Alamar blue® assay was detected by SpectraMax M5 microplate reader (Molecular Device Inc. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. SpectraMax® M5e. 0 out of. With optimized reagents, option to operate the. Methods Preparation of reagents and standards. Figure 1. Bio-Tek BioTek uQuant Microplate Reader Complete w WARRANTY $3,450. Designed to provide the simplicity, flexibility and power required for advanced data analysis. 2. Plate Shaking. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. used. begin reading microplates with seven mouse clicks. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. SpectraMax Lmicroplate luminometer. As a generous estimate, you might consider 300 W (at 120 Volts) per Spectramax reader, plus another 300 W for the Computer. 59 6. SpectraMax M3 M4 M5 M5e SpectraTest FL1 revA. SpectraMax iD3 Multi-Mode Microplate Reader; Methods Cell treatment. SoftMax Pro Software has been integrated by many leading robotics andFor readers without a cuvette port, simply check the box next to ‘PathCheck’. -Wide range of applications -Five preferred detection modes -Maximum signal with lowest background The dual monochromator optics allow the widest range of. For readers without a cuvette port, these options are not shown in the software. The following document is intended to demonstrate setup of this instrument. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. Sensitive fluorescent quantitation of DNA with the Quant-iT. Insert the filled microplate into the drawer, matching well A1 with position A1 in the drawer. Five preferred detection modes. Next, add 10 μl of the MTT labeling reagent (5 mg/mL) to each well for 4 h. Results shown are from the SpectraMax M5 microplate reader, but similar results are obtained with other Molecular Devices readers with fluorescence detection mode (data not shown). Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. Spectramax M5 microplate reader: Molecular Devices: N/A:. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. This kit is optimized for Molecular Devices SpectraMax microplate readers with a preconfigured protocol provided in SoftMax Pro Software for simplified data acquisition and analysis. 04 to 3. SpectraMax® M5. Linear, Orbital Slow, and Orbital Fast. Our absorbance plate readers feature our PathCheck Sensor technology and. The spectrophotometric. The percentage of viable cells for each treatment group was calculated by adjusting the untreated control group to 100%. View the Molecular Devices website. AquaMax® 2000. Microplate Reader Documentation Version No. Add to Cart. FlexStation® 3 Multi-Mode Microplate Reader. As the ratio of ADP:ATP increases, the proportion of bound tracer vs. Here the delta mP was 336, and Z’ factor was 0. 2. Description: The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader. 3. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. E Models Only) 69. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. book Page 7 Wednesday, July 7, 2010 2:09 PM. In SOFTmax PRO, set up a plate section for an excitation scan with no cutoff filter and use the medium PMT setting. After 24 h drug treatment, cells were lysed by RIPA buffer (Life Technologies Inc, US). B: Selection of Excitation Wavelength. The SpectraMax L Microplate Reader was set up for endpoint reading with an inte-gration time of 1 second or 0. Conclusions We report a peptide-induced super-assembly of enzyme-containing MOFs for programmed enzyme. 1 Version 7. 7 cells and HEK293T cells were seeded at the density of 2 × 105 cells per well in a 24-well plate overnight. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. The readings with excitation at 540 nm and emission at 600 nm represented the amount of phosphorylated IκB-α in the cells. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. upgradeable with a wide range of high performance capabilities ideal for life science research and drug. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode MicroplateReaderaredualmonochromator,multi-detectioninstrumentswithatriple-mode cuvetteportanda6-wellto384-wellplatereadcapability. The absorbance values at 450 nm were determined by the SpectraMax M5 microplate reader. A control experiment of the activity of the proposed enzyme/ZIF-8A particles without peptides against protease digestion was also performed. Add to Cart. 67. FlexStation™ II. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. on the SpectraMax Multi-Mode Microplate Readers by issuing a single. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Thank you!. SDS-PAGE Assay. Microplate Readers include the M3, M4, M5, and M5e models. We would like to show you a description here but the site won’t allow us. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. ® reader to the computer. 5. The SPECTRAmax™ PLUS microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific pro-teins, nucleic acids, and other molecules across a wide range of concentrations. The following document is intended to demonstrate setup of this instrument. The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi-wavelength and. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence microplate readers. Page 28 Chapter 5: Read Modes and Read Types 1. View the Molecular Devices website. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. Choose from a three- (M3), four- (M4), or0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. For a higher . The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. In . 96-1536. 2 only). Analyst, M5, Flex 3, and SpectraMax L readers work differently. book Page 7 Wednesday, July 7, 2010 2:09 PM. Preparation of Calibration Standards Calibration Standard (% Phosphorylated) µL Peptide Stock µL Phospho-peptide Stock 0 100 0 12. Examples: “Press carefully to engage,” “ Do not. SoftMax Pro 6 User Guide is a comprehensive manual for the latest version of the software that allows you to control and analyze data from microplate readers. AquaMax® 2000. 0. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. SpectraMax® M4. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. 0. 95. The integration time suggested is 5-10 sec. Preparation of the peritoneal macrophages The peritoneal macrophages were obtained from the mice after intraperitoneally (i. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Microplate Readers Biotek Synergy H1 Hybrid Microplate Reader. 54 1. Molecular Devices Corporation. 305 reference level saturation. These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-ModePlate chamber design of the SpectraMax category 1 microplate readers. Italic and boldface type are used for emphasis. Figure 4: Transcreener ADP. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, SoftMax Pro Software, SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax M5/M5e reader. SpectraMax® ABS Plus. The acceptance. SoftMax® Pro Software is the most published microplate reader control and data analysis software in the industry. Microbiota DNA was. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. We specialize in the full suite of AKTA™ FPLC systems, Molecular Devices SpectraMax™ microplate readers, BioTek microplate washers, YMC (formerly LEWA) Process Technologies systems, ChromaCon Contichrom® instruments, and. Users can select from top or bottom read modes for improved sensitivity for solution and cell-based assays. • SpectraMax M5 Multi-Mode Microplate Reader Figure 2. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. Maximum signal with lowest background. The SpectraMax M2 and M2e are versatile and reliable. Absorbance was read on a Spectramax M5 Microplate reader at 450 nm. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Western blotting analysis. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. Then, 100 μL MTT lysis buffer was added to each well and incubated for 16 h. Molecular Devices Spectramax M5e Multi Mode Microplate Reader; Related products. Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates. Microplate Capacity. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. Lower limits of detection for ATP determined using the ATPlite . The CellTiter-Glo assay from Promega uses luciferase. FlexStation™ I. The dual monochromator optics allow the widest. SpectraMax® M4. Shimadzu 2450 UV–Vis spectrophotometer and SpectraMax M5 microplate reader were used to perform the optical characterization and measurements. Read one sample or up to 384 inThe SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). 7 cells (6 × 10 5 cells/well in 6-well plates) were pretreated with of 3,3′,4,5′-TMS or 3,4′,5-TMS at 10 and 50 µM for 4 h, followed by the addition of LPS (1 µg/mL) for 12 h. Trilevel commercial control reagents (StanbioThe SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual- monochromator microplate reader platform offering a wide range of. ATP concentration (M) Average RLU SpectraMax L SpectraMax i3x (cartridge) SpectraMax Mini SpectraMax iD5 SpectraMax i3x (onboard) SpectraMax M5eThe PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. 5 25 75 25 50 50 50 100 0 100 结合反应 Table 2. Here, we illustrate how the SpectraMax iD5 reader, in combination with SoftMax ® Pro Software, is used to quantitate a cellular protein sample with two different kits, Pierce BCA Protein Assay Kit and Pierce Rapid. 2. 00. #M5) SpectraMax® L Microplate Reader (cat. fluorescence polarization, trf, fret, igg quantitation, elisa, western blot, spectramax i3x, spectramax id5 multi-mode microplate reader, spectramax id5, spectramax paradigm, spectramax m5, spectramax m5 multi-mode microplate reader, high-throughput igg quantitation, mab igg titer, softmax pro, valita titer, valitatiter. TNF-alpha quantification down to low picomolar levels on the SpectraMax Paradigm Platform. The SpectraMax® ABS Plus Microplate Reader can run . Are you using the correct Microplate Adapters? For the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. When the user applies a set temperature of 37°C, the top is heated to 37. Bookings are free, but you will need to use iLab to book. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini EM Reader, users never have to worry about not having the right set of filters. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore ([email protected] or greater. Add to Cart. Biotek FLx800 Fluorescence Microplate Reader; Related products-20%. RAW 264. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Z’-LYTE® Assay using the Z'-LYTE® Tyr6 kit. The cells were incubated with compounds at the indicated doses for 1 h. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. Temperature variation ± 0. Music: Cylinder Six by Chris Zabriskie is licensed under a Creative Commons Attribution license (microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm. book Page 1 Wednesday, July 7, 2010 2:09 PMThe SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Put 200 µL sample into one or more wells of a microplate and 200 µL buffer or solvent into one or more other wells. With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. Fluorescence Microplate Readers. SpectraMax M5 Multi-Mode Microplate Reader. for ValitaTiter Plus standards read on Molecular Devices readers. Figure 1. Products : SpectraMax M4 reader, FilterMax F5 reader, SpectraMax Paradigm reader, SpectraMax M5/M5e reader Do you know any applications for TRF/HTRF that differ from Fluorescence Polarization? TR-FRET assays are well-suited for protein:protein interactions. view more . The PBS solution (10. Standard SDS-PAGE was performed with a 10% (w/v) separating gel and a 4% stacking gel. Ltd. LMax II 384 Microplate Luminometer: Integration time = 1 second, Settling time = off, Autoread = off. Between steps, reactions were rinsed 3 times with TBS + 0. D. Readings were taken using a UV–Vis Spectramax M5 ® microplate reader (Molecular Device, USA). It is important to determine the excitation and emission wavelengths that provide the best dynamic range for the assay, since these may differ somewhat from those used with filter-based plate readers. 1 second. How do I set up Hellma Cuvette validation set recertification? Send Hellma cuvette standards to Hellma. 3 mg. 26 1. Molecular Devices Spectramax M5 Microplate Reader; Related products. Both readers feature an 8-channel read head for fast reads and.